YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration
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YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration. / Yui, Shiro; Azzolin, Luca; Maimets, Martti; Pedersen, Marianne Terndrup; Fordham, Robert P.; Hansen, Stine L.; Larsen, Hjalte L.; Guiu, Jordi; Alves, Mariana R.P.; Rundsten, Carsten F.; Johansen, Jens V.; Li, Yuan; Madsen, Chris D.; Nakamura, Tetsuya; Watanabe, Mamoru; Nielsen, Ole H.; Schweiger, Pawel J.; Piccolo, Stefano; Jensen, Kim B.
In: Cell Stem Cell, Vol. 22, No. 1, 01.01.2018, p. 35-49. e7.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration
AU - Yui, Shiro
AU - Azzolin, Luca
AU - Maimets, Martti
AU - Pedersen, Marianne Terndrup
AU - Fordham, Robert P.
AU - Hansen, Stine L.
AU - Larsen, Hjalte L.
AU - Guiu, Jordi
AU - Alves, Mariana R.P.
AU - Rundsten, Carsten F.
AU - Johansen, Jens V.
AU - Li, Yuan
AU - Madsen, Chris D.
AU - Nakamura, Tetsuya
AU - Watanabe, Mamoru
AU - Nielsen, Ole H.
AU - Schweiger, Pawel J.
AU - Piccolo, Stefano
AU - Jensen, Kim B.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Tissue regeneration requires dynamic cellular adaptation to the wound environment. It is currently unclear how this is orchestrated at the cellular level and how cell fate is affected by severe tissue damage. Here we dissect cell fate transitions during colonic regeneration in a mouse dextran sulfate sodium (DSS) colitis model, and we demonstrate that the epithelium is transiently reprogrammed into a primitive state. This is characterized by de novo expression of fetal markers as well as suppression of markers for adult stem and differentiated cells. The fate change is orchestrated by remodeling the extracellular matrix (ECM), increased FAK/Src signaling, and ultimately YAP/TAZ activation. In a defined cell culture system recapitulating the extracellular matrix remodeling observed in vivo, we show that a collagen 3D matrix supplemented with Wnt ligands is sufficient to sustain endogenous YAP/TAZ and induce conversion of cell fate. This provides a simple model for tissue regeneration, implicating cellular reprogramming as an essential element. The mechanism that governs tissue regeneration following severe damage to the colonic epithelium remains poorly understood. Jensen and colleagues show that the colonic epithelium undergoes a profound reprogramming into a more primitive state with fetal-like properties. Moreover, they demonstrate that YAP and TAZ operate as essential mechano-sensors during tissue reprogramming.
AB - Tissue regeneration requires dynamic cellular adaptation to the wound environment. It is currently unclear how this is orchestrated at the cellular level and how cell fate is affected by severe tissue damage. Here we dissect cell fate transitions during colonic regeneration in a mouse dextran sulfate sodium (DSS) colitis model, and we demonstrate that the epithelium is transiently reprogrammed into a primitive state. This is characterized by de novo expression of fetal markers as well as suppression of markers for adult stem and differentiated cells. The fate change is orchestrated by remodeling the extracellular matrix (ECM), increased FAK/Src signaling, and ultimately YAP/TAZ activation. In a defined cell culture system recapitulating the extracellular matrix remodeling observed in vivo, we show that a collagen 3D matrix supplemented with Wnt ligands is sufficient to sustain endogenous YAP/TAZ and induce conversion of cell fate. This provides a simple model for tissue regeneration, implicating cellular reprogramming as an essential element. The mechanism that governs tissue regeneration following severe damage to the colonic epithelium remains poorly understood. Jensen and colleagues show that the colonic epithelium undergoes a profound reprogramming into a more primitive state with fetal-like properties. Moreover, they demonstrate that YAP and TAZ operate as essential mechano-sensors during tissue reprogramming.
KW - Intestinal stem cells
KW - Mechano-sensing
KW - Regeneration
KW - Reprogramming
KW - YAP/TAZ
U2 - 10.1016/j.stem.2017.11.001
DO - 10.1016/j.stem.2017.11.001
M3 - Journal article
C2 - 29249464
AN - SCOPUS:85039042378
VL - 22
SP - 35-49. e7
JO - Cell Stem Cell
JF - Cell Stem Cell
SN - 1934-5909
IS - 1
ER -
ID: 188113397