Tus-Ter as a tool to study site-specific DNA replication perturbation in eukaryotes

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Standard

Tus-Ter as a tool to study site-specific DNA replication perturbation in eukaryotes. / Larsen, Nicolai B; Hickson, Ian D; Mankouri, Hocine W.

In: Cell Cycle, Vol. 13, No. 19, 01.10.2014, p. 2994-8.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Larsen, NB, Hickson, ID & Mankouri, HW 2014, 'Tus-Ter as a tool to study site-specific DNA replication perturbation in eukaryotes', Cell Cycle, vol. 13, no. 19, pp. 2994-8. https://doi.org/10.4161/15384101.2014.958912

APA

Larsen, N. B., Hickson, I. D., & Mankouri, H. W. (2014). Tus-Ter as a tool to study site-specific DNA replication perturbation in eukaryotes. Cell Cycle, 13(19), 2994-8. https://doi.org/10.4161/15384101.2014.958912

Vancouver

Larsen NB, Hickson ID, Mankouri HW. Tus-Ter as a tool to study site-specific DNA replication perturbation in eukaryotes. Cell Cycle. 2014 Oct 1;13(19):2994-8. https://doi.org/10.4161/15384101.2014.958912

Author

Larsen, Nicolai B ; Hickson, Ian D ; Mankouri, Hocine W. / Tus-Ter as a tool to study site-specific DNA replication perturbation in eukaryotes. In: Cell Cycle. 2014 ; Vol. 13, No. 19. pp. 2994-8.

Bibtex

@article{f8bfbc6b4def468f8a041b9bf0637148,
title = "Tus-Ter as a tool to study site-specific DNA replication perturbation in eukaryotes",
abstract = "The high-affinity binding of the Tus protein to specific 21-bp sequences, called Ter, causes site-specific, and polar, DNA replication fork arrest in E coli. The Tus-Ter complex serves to coordinate DNA replication with chromosome segregation in this organism. A number of recent and ongoing studies have demonstrated that Tus-Ter can be used as a heterologous tool to generate site-specific perturbation of DNA replication when reconstituted in eukaryotes. Here, we review these recent findings and explore the molecular mechanism by which Tus-Ter mediates replication fork (RF) arrest in the budding yeast, S. cerevisiae. We propose that Tus-Ter is a versatile, genetically tractable, and regulatable RF blocking system that can be utilized for disrupting DNA replication in a diverse range of host cells.",
author = "Larsen, {Nicolai B} and Hickson, {Ian D} and Mankouri, {Hocine W}",
year = "2014",
month = oct,
day = "1",
doi = "10.4161/15384101.2014.958912",
language = "English",
volume = "13",
pages = "2994--8",
journal = "Cell Cycle",
issn = "1538-4101",
publisher = "Taylor & Francis",
number = "19",

}

RIS

TY - JOUR

T1 - Tus-Ter as a tool to study site-specific DNA replication perturbation in eukaryotes

AU - Larsen, Nicolai B

AU - Hickson, Ian D

AU - Mankouri, Hocine W

PY - 2014/10/1

Y1 - 2014/10/1

N2 - The high-affinity binding of the Tus protein to specific 21-bp sequences, called Ter, causes site-specific, and polar, DNA replication fork arrest in E coli. The Tus-Ter complex serves to coordinate DNA replication with chromosome segregation in this organism. A number of recent and ongoing studies have demonstrated that Tus-Ter can be used as a heterologous tool to generate site-specific perturbation of DNA replication when reconstituted in eukaryotes. Here, we review these recent findings and explore the molecular mechanism by which Tus-Ter mediates replication fork (RF) arrest in the budding yeast, S. cerevisiae. We propose that Tus-Ter is a versatile, genetically tractable, and regulatable RF blocking system that can be utilized for disrupting DNA replication in a diverse range of host cells.

AB - The high-affinity binding of the Tus protein to specific 21-bp sequences, called Ter, causes site-specific, and polar, DNA replication fork arrest in E coli. The Tus-Ter complex serves to coordinate DNA replication with chromosome segregation in this organism. A number of recent and ongoing studies have demonstrated that Tus-Ter can be used as a heterologous tool to generate site-specific perturbation of DNA replication when reconstituted in eukaryotes. Here, we review these recent findings and explore the molecular mechanism by which Tus-Ter mediates replication fork (RF) arrest in the budding yeast, S. cerevisiae. We propose that Tus-Ter is a versatile, genetically tractable, and regulatable RF blocking system that can be utilized for disrupting DNA replication in a diverse range of host cells.

U2 - 10.4161/15384101.2014.958912

DO - 10.4161/15384101.2014.958912

M3 - Journal article

C2 - 25486560

VL - 13

SP - 2994

EP - 2998

JO - Cell Cycle

JF - Cell Cycle

SN - 1538-4101

IS - 19

ER -

ID: 129809936