The Escherichia coli Tus-Ter replication fork barrier causes site-specific DNA replication perturbation in yeast

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Standard

The Escherichia coli Tus-Ter replication fork barrier causes site-specific DNA replication perturbation in yeast. / Larsen, Nicolai B; Sass, Ehud; Suski, Catherine; Mankouri, Hocine W; Hickson, Ian D.

In: Nature Communications, Vol. 5, 3574, 2014, p. 1-10.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Larsen, NB, Sass, E, Suski, C, Mankouri, HW & Hickson, ID 2014, 'The Escherichia coli Tus-Ter replication fork barrier causes site-specific DNA replication perturbation in yeast', Nature Communications, vol. 5, 3574, pp. 1-10. https://doi.org/10.1038/ncomms4574

APA

Larsen, N. B., Sass, E., Suski, C., Mankouri, H. W., & Hickson, I. D. (2014). The Escherichia coli Tus-Ter replication fork barrier causes site-specific DNA replication perturbation in yeast. Nature Communications, 5, 1-10. [3574]. https://doi.org/10.1038/ncomms4574

Vancouver

Larsen NB, Sass E, Suski C, Mankouri HW, Hickson ID. The Escherichia coli Tus-Ter replication fork barrier causes site-specific DNA replication perturbation in yeast. Nature Communications. 2014;5:1-10. 3574. https://doi.org/10.1038/ncomms4574

Author

Larsen, Nicolai B ; Sass, Ehud ; Suski, Catherine ; Mankouri, Hocine W ; Hickson, Ian D. / The Escherichia coli Tus-Ter replication fork barrier causes site-specific DNA replication perturbation in yeast. In: Nature Communications. 2014 ; Vol. 5. pp. 1-10.

Bibtex

@article{c7e3667d2da34e3093a655bef1211bb4,
title = "The Escherichia coli Tus-Ter replication fork barrier causes site-specific DNA replication perturbation in yeast",
abstract = "Replication fork (RF) pausing occurs at both 'programmed' sites and non-physiological barriers (for example, DNA adducts). Programmed RF pausing is required for site-specific DNA replication termination in Escherichia coli, and this process requires the binding of the polar terminator protein, Tus, to specific DNA sequences called Ter. Here, we demonstrate that Tus-Ter modules also induce polar RF pausing when engineered into the Saccharomyces cerevisiae genome. This heterologous RF barrier is distinct from a number of previously characterized, protein-mediated, RF pause sites in yeast, as it is neither Tof1-dependent nor counteracted by the Rrm3 helicase. Although the yeast replisome can overcome RF pausing at Tus-Ter modules, this event triggers site-specific homologous recombination that requires the RecQ helicase, Sgs1, for its timely resolution. We propose that Tus-Ter can be utilized as a versatile, site-specific, heterologous DNA replication-perturbing system, with a variety of potential applications.",
author = "Larsen, {Nicolai B} and Ehud Sass and Catherine Suski and Mankouri, {Hocine W} and Hickson, {Ian D}",
year = "2014",
doi = "10.1038/ncomms4574",
language = "English",
volume = "5",
pages = "1--10",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - The Escherichia coli Tus-Ter replication fork barrier causes site-specific DNA replication perturbation in yeast

AU - Larsen, Nicolai B

AU - Sass, Ehud

AU - Suski, Catherine

AU - Mankouri, Hocine W

AU - Hickson, Ian D

PY - 2014

Y1 - 2014

N2 - Replication fork (RF) pausing occurs at both 'programmed' sites and non-physiological barriers (for example, DNA adducts). Programmed RF pausing is required for site-specific DNA replication termination in Escherichia coli, and this process requires the binding of the polar terminator protein, Tus, to specific DNA sequences called Ter. Here, we demonstrate that Tus-Ter modules also induce polar RF pausing when engineered into the Saccharomyces cerevisiae genome. This heterologous RF barrier is distinct from a number of previously characterized, protein-mediated, RF pause sites in yeast, as it is neither Tof1-dependent nor counteracted by the Rrm3 helicase. Although the yeast replisome can overcome RF pausing at Tus-Ter modules, this event triggers site-specific homologous recombination that requires the RecQ helicase, Sgs1, for its timely resolution. We propose that Tus-Ter can be utilized as a versatile, site-specific, heterologous DNA replication-perturbing system, with a variety of potential applications.

AB - Replication fork (RF) pausing occurs at both 'programmed' sites and non-physiological barriers (for example, DNA adducts). Programmed RF pausing is required for site-specific DNA replication termination in Escherichia coli, and this process requires the binding of the polar terminator protein, Tus, to specific DNA sequences called Ter. Here, we demonstrate that Tus-Ter modules also induce polar RF pausing when engineered into the Saccharomyces cerevisiae genome. This heterologous RF barrier is distinct from a number of previously characterized, protein-mediated, RF pause sites in yeast, as it is neither Tof1-dependent nor counteracted by the Rrm3 helicase. Although the yeast replisome can overcome RF pausing at Tus-Ter modules, this event triggers site-specific homologous recombination that requires the RecQ helicase, Sgs1, for its timely resolution. We propose that Tus-Ter can be utilized as a versatile, site-specific, heterologous DNA replication-perturbing system, with a variety of potential applications.

U2 - 10.1038/ncomms4574

DO - 10.1038/ncomms4574

M3 - Journal article

C2 - 24705096

VL - 5

SP - 1

EP - 10

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 3574

ER -

ID: 108776990