Rmi1 stimulates decatenation of double Holliday junctions during dissolution by Sgs1-Top3
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Rmi1 stimulates decatenation of double Holliday junctions during dissolution by Sgs1-Top3. / Cejka, Petr; Plank, Jody L; Bachrati, Csanad Z; Hickson, Ian D; Kowalczykowski, Stephen C.
In: Nature Structural & Molecular Biology, Vol. 17, No. 11, 01.11.2010, p. 1377-82.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Rmi1 stimulates decatenation of double Holliday junctions during dissolution by Sgs1-Top3
AU - Cejka, Petr
AU - Plank, Jody L
AU - Bachrati, Csanad Z
AU - Hickson, Ian D
AU - Kowalczykowski, Stephen C
PY - 2010/11/1
Y1 - 2010/11/1
N2 - A double Holliday junction (dHJ) is a central intermediate of homologous recombination that can be processed to yield crossover or non-crossover recombination products. To preserve genomic integrity, cells possess mechanisms to avoid crossing over. We show that Saccharomyces cerevisiae Sgs1 and Top3 proteins are sufficient to migrate and disentangle a dHJ to produce exclusively non-crossover recombination products, in a reaction termed "dissolution." We show that Rmi1 stimulates dHJ dissolution at low Sgs1-Top3 protein concentrations, although it has no effect on the initial rate of Holliday junction (HJ) migration. Rmi1 serves to stimulate DNA decatenation, removing the last linkages between the repaired and template DNA molecules. Dissolution of a dHJ is a highly efficient and concerted alternative to nucleolytic resolution that prevents crossing over of chromosomes during recombinational DNA repair in mitotic cells and thereby contributes to genomic integrity.
AB - A double Holliday junction (dHJ) is a central intermediate of homologous recombination that can be processed to yield crossover or non-crossover recombination products. To preserve genomic integrity, cells possess mechanisms to avoid crossing over. We show that Saccharomyces cerevisiae Sgs1 and Top3 proteins are sufficient to migrate and disentangle a dHJ to produce exclusively non-crossover recombination products, in a reaction termed "dissolution." We show that Rmi1 stimulates dHJ dissolution at low Sgs1-Top3 protein concentrations, although it has no effect on the initial rate of Holliday junction (HJ) migration. Rmi1 serves to stimulate DNA decatenation, removing the last linkages between the repaired and template DNA molecules. Dissolution of a dHJ is a highly efficient and concerted alternative to nucleolytic resolution that prevents crossing over of chromosomes during recombinational DNA repair in mitotic cells and thereby contributes to genomic integrity.
KW - DNA, Cruciform
KW - DNA, Fungal
KW - DNA-Binding Proteins
KW - RecQ Helicases
KW - Saccharomyces cerevisiae
KW - Saccharomyces cerevisiae Proteins
U2 - 10.1038/nsmb.1919
DO - 10.1038/nsmb.1919
M3 - Journal article
C2 - 20935631
VL - 17
SP - 1377
EP - 1382
JO - Nature Structural and Molecular Biology
JF - Nature Structural and Molecular Biology
SN - 1545-9993
IS - 11
ER -
ID: 33489825