Regulation of the golgi apparatus by p38 and JNK kinases during cellular stress responses
Research output: Contribution to journal › Journal article › Research › peer-review
Documents
- Regulation of the Golgi Apparatus by p38 and JNK Kinases during Cellular Stress Responses
Final published version, 11 MB, PDF document
p38 and c-Jun N-terninal kinase (JNK) are activated in response to acute stress and inflammatory signals. Through modification of a plethora of substrates, these kinases profoundly re-shape cellular physiology for the optimal response to a harmful environment and/or an inflammatory state. Here, we utilized phospho-proteomics to identify several hundred substrates for both kinases. Our results indicate that the scale of signaling from p38 and JNK are of a similar magnitude. Among the many new targets, we highlight the regulation of the transcriptional regulators grb10-interacting GYF protein 1 and 2 (GIGYF1/2) by p38-dependent MAP kinase-activated protein kinase 2 (MK2) phosphorylation and 14–3–3 binding. We also show that the Golgi apparatus contains numerous substrates, and is a major target for regulation by p38 and JNK. When activated, these kinases mediate structural rearrangement of the Golgi apparatus, which positively affects protein flux through the secretory system. Our work expands on our knowledge about p38 and JNK signaling with important biological ramifications.
Original language | English |
---|---|
Article number | 9595 |
Journal | International Journal of Molecular Sciences |
Volume | 22 |
Issue number | 17 |
Number of pages | 23 |
ISSN | 1661-6596 |
DOIs | |
Publication status | Published - 2021 |
Bibliographical note
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
- GIGYF, JNK, P38, Phosphorylation, Stress signaling, Translation and Golgi
Research areas
Number of downloads are based on statistics from Google Scholar and www.ku.dk
ID: 280064152