Reconstitution of anaphase DNA bridge recognition and disjunction

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Reconstitution of anaphase DNA bridge recognition and disjunction. / Sarlós, Kata; Biebricher, Andreas S.; Bizard, Anna H.; Bakx, Julia A.M.; Ferreté-Bonastre, Anna G.; Modesti, Mauro; Paramasivam, Manikandan; Yao, Qi; Peterman, Erwin J.G.; Wuite, Gijs J.L.; Hickson, Ian D.

In: Nature Structural and Molecular Biology, Vol. 25, No. 9, 2018, p. 868-876.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Sarlós, K, Biebricher, AS, Bizard, AH, Bakx, JAM, Ferreté-Bonastre, AG, Modesti, M, Paramasivam, M, Yao, Q, Peterman, EJG, Wuite, GJL & Hickson, ID 2018, 'Reconstitution of anaphase DNA bridge recognition and disjunction', Nature Structural and Molecular Biology, vol. 25, no. 9, pp. 868-876. https://doi.org/10.1038/s41594-018-0123-8

APA

Sarlós, K., Biebricher, A. S., Bizard, A. H., Bakx, J. A. M., Ferreté-Bonastre, A. G., Modesti, M., Paramasivam, M., Yao, Q., Peterman, E. J. G., Wuite, G. J. L., & Hickson, I. D. (2018). Reconstitution of anaphase DNA bridge recognition and disjunction. Nature Structural and Molecular Biology, 25(9), 868-876. https://doi.org/10.1038/s41594-018-0123-8

Vancouver

Sarlós K, Biebricher AS, Bizard AH, Bakx JAM, Ferreté-Bonastre AG, Modesti M et al. Reconstitution of anaphase DNA bridge recognition and disjunction. Nature Structural and Molecular Biology. 2018;25(9):868-876. https://doi.org/10.1038/s41594-018-0123-8

Author

Sarlós, Kata ; Biebricher, Andreas S. ; Bizard, Anna H. ; Bakx, Julia A.M. ; Ferreté-Bonastre, Anna G. ; Modesti, Mauro ; Paramasivam, Manikandan ; Yao, Qi ; Peterman, Erwin J.G. ; Wuite, Gijs J.L. ; Hickson, Ian D. / Reconstitution of anaphase DNA bridge recognition and disjunction. In: Nature Structural and Molecular Biology. 2018 ; Vol. 25, No. 9. pp. 868-876.

Bibtex

@article{4bc3445f53ca469c9bfbe28e5747c4bb,
title = "Reconstitution of anaphase DNA bridge recognition and disjunction",
abstract = "Faithful chromosome segregation requires that the sister chromatids be disjoined completely. Defective disjunction can lead to the persistence of histone-free threads of DNA known as ultra-fine bridges (UFBs) that connect the separating sister DNA molecules during anaphase. UFBs arise at specific genomic loci and can only be visualized by detection of associated proteins such as PICH, BLM, topoisomerase IIIα, and RPA. However, it remains unknown how these proteins work together to promote UFB processing. We used a combination of ensemble biochemistry and new single-molecule assays to reconstitute key steps of UFB recognition and processing by these human proteins in vitro. We discovered characteristic patterns of hierarchical recruitment and coordinated biochemical activities that were specific for DNA structures modeling UFBs arising at either centromeres or common fragile sites. Our results describe a mechanistic model for how unresolved DNA replication structures are processed by DNA-structure-specific binding factors in mitosis to prevent pathological chromosome nondisjunction.",
author = "Kata Sarl{\'o}s and Biebricher, {Andreas S.} and Bizard, {Anna H.} and Bakx, {Julia A.M.} and Ferret{\'e}-Bonastre, {Anna G.} and Mauro Modesti and Manikandan Paramasivam and Qi Yao and Peterman, {Erwin J.G.} and Wuite, {Gijs J.L.} and Hickson, {Ian D.}",
year = "2018",
doi = "10.1038/s41594-018-0123-8",
language = "English",
volume = "25",
pages = "868--876",
journal = "Nature Structural and Molecular Biology",
issn = "1545-9993",
publisher = "nature publishing group",
number = "9",

}

RIS

TY - JOUR

T1 - Reconstitution of anaphase DNA bridge recognition and disjunction

AU - Sarlós, Kata

AU - Biebricher, Andreas S.

AU - Bizard, Anna H.

AU - Bakx, Julia A.M.

AU - Ferreté-Bonastre, Anna G.

AU - Modesti, Mauro

AU - Paramasivam, Manikandan

AU - Yao, Qi

AU - Peterman, Erwin J.G.

AU - Wuite, Gijs J.L.

AU - Hickson, Ian D.

PY - 2018

Y1 - 2018

N2 - Faithful chromosome segregation requires that the sister chromatids be disjoined completely. Defective disjunction can lead to the persistence of histone-free threads of DNA known as ultra-fine bridges (UFBs) that connect the separating sister DNA molecules during anaphase. UFBs arise at specific genomic loci and can only be visualized by detection of associated proteins such as PICH, BLM, topoisomerase IIIα, and RPA. However, it remains unknown how these proteins work together to promote UFB processing. We used a combination of ensemble biochemistry and new single-molecule assays to reconstitute key steps of UFB recognition and processing by these human proteins in vitro. We discovered characteristic patterns of hierarchical recruitment and coordinated biochemical activities that were specific for DNA structures modeling UFBs arising at either centromeres or common fragile sites. Our results describe a mechanistic model for how unresolved DNA replication structures are processed by DNA-structure-specific binding factors in mitosis to prevent pathological chromosome nondisjunction.

AB - Faithful chromosome segregation requires that the sister chromatids be disjoined completely. Defective disjunction can lead to the persistence of histone-free threads of DNA known as ultra-fine bridges (UFBs) that connect the separating sister DNA molecules during anaphase. UFBs arise at specific genomic loci and can only be visualized by detection of associated proteins such as PICH, BLM, topoisomerase IIIα, and RPA. However, it remains unknown how these proteins work together to promote UFB processing. We used a combination of ensemble biochemistry and new single-molecule assays to reconstitute key steps of UFB recognition and processing by these human proteins in vitro. We discovered characteristic patterns of hierarchical recruitment and coordinated biochemical activities that were specific for DNA structures modeling UFBs arising at either centromeres or common fragile sites. Our results describe a mechanistic model for how unresolved DNA replication structures are processed by DNA-structure-specific binding factors in mitosis to prevent pathological chromosome nondisjunction.

U2 - 10.1038/s41594-018-0123-8

DO - 10.1038/s41594-018-0123-8

M3 - Journal article

C2 - 30177760

AN - SCOPUS:85052987271

VL - 25

SP - 868

EP - 876

JO - Nature Structural and Molecular Biology

JF - Nature Structural and Molecular Biology

SN - 1545-9993

IS - 9

ER -

ID: 209743736