PICH promotes mitotic chromosome segregation: Identification of a novel role in rDNA disjunction
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PICH promotes mitotic chromosome segregation : Identification of a novel role in rDNA disjunction. / Nielsen, Christian Friberg; Hickson, Ian D.
In: Cell Cycle, Vol. 15, No. 20, 17.10.2016, p. 2704-2711.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - PICH promotes mitotic chromosome segregation
T2 - Identification of a novel role in rDNA disjunction
AU - Nielsen, Christian Friberg
AU - Hickson, Ian D
PY - 2016/10/17
Y1 - 2016/10/17
N2 - PICH is an SNF2-family DNA translocase that appears to play a role specifically in mitosis. Characterization of PICH in human cells led to the initial discovery of "ultra-fine DNA bridges" (UFBs) that connect the 2 segregating DNA masses in the anaphase of mitosis. These bridge structures, which arise from specific regions of the genome, are a normal feature of anaphase but had escaped detection previously because they do not stain with commonly used DNA dyes. Nevertheless, UFBs are important for genome maintenance because defects in UFB resolution can lead to cytokinesis failure. We reported recently that PICH stimulates the unlinking (decatenation) of entangled DNA by Topoisomerase IIα (Topo IIα), and is important for the resolution of UFBs. We also demonstrated that PICH and Topo IIα co-localize at the rDNA (rDNA). In this Extra View article, we discuss the mitotic roles of PICH and explore further the role of PICH in the timely segregation of the rDNA locus.
AB - PICH is an SNF2-family DNA translocase that appears to play a role specifically in mitosis. Characterization of PICH in human cells led to the initial discovery of "ultra-fine DNA bridges" (UFBs) that connect the 2 segregating DNA masses in the anaphase of mitosis. These bridge structures, which arise from specific regions of the genome, are a normal feature of anaphase but had escaped detection previously because they do not stain with commonly used DNA dyes. Nevertheless, UFBs are important for genome maintenance because defects in UFB resolution can lead to cytokinesis failure. We reported recently that PICH stimulates the unlinking (decatenation) of entangled DNA by Topoisomerase IIα (Topo IIα), and is important for the resolution of UFBs. We also demonstrated that PICH and Topo IIα co-localize at the rDNA (rDNA). In this Extra View article, we discuss the mitotic roles of PICH and explore further the role of PICH in the timely segregation of the rDNA locus.
U2 - 10.1080/15384101.2016.1222336
DO - 10.1080/15384101.2016.1222336
M3 - Journal article
C2 - 27565185
VL - 15
SP - 2704
EP - 2711
JO - Cell Cycle
JF - Cell Cycle
SN - 1538-4101
IS - 20
ER -
ID: 166272486