Human topoisomerase IIIalpha is a single-stranded DNA decatenase that is stimulated by BLM and RMI1

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Human topoisomerase IIIalpha is a single-stranded DNA decatenase that is stimulated by BLM and RMI1. / Yang, Jay; Bachrati, Csanad Z; Ou, Jiongwen; Hickson, Ian David; Brown, Grant W.

In: Journal of Biological Chemistry, Vol. 285, No. 28, 2010, p. 21426-36.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Yang, J, Bachrati, CZ, Ou, J, Hickson, ID & Brown, GW 2010, 'Human topoisomerase IIIalpha is a single-stranded DNA decatenase that is stimulated by BLM and RMI1', Journal of Biological Chemistry, vol. 285, no. 28, pp. 21426-36. https://doi.org/10.1074/jbc.M110.123216

APA

Yang, J., Bachrati, C. Z., Ou, J., Hickson, I. D., & Brown, G. W. (2010). Human topoisomerase IIIalpha is a single-stranded DNA decatenase that is stimulated by BLM and RMI1. Journal of Biological Chemistry, 285(28), 21426-36. https://doi.org/10.1074/jbc.M110.123216

Vancouver

Yang J, Bachrati CZ, Ou J, Hickson ID, Brown GW. Human topoisomerase IIIalpha is a single-stranded DNA decatenase that is stimulated by BLM and RMI1. Journal of Biological Chemistry. 2010;285(28):21426-36. https://doi.org/10.1074/jbc.M110.123216

Author

Yang, Jay ; Bachrati, Csanad Z ; Ou, Jiongwen ; Hickson, Ian David ; Brown, Grant W. / Human topoisomerase IIIalpha is a single-stranded DNA decatenase that is stimulated by BLM and RMI1. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 28. pp. 21426-36.

Bibtex

@article{f7d1d4b0958f11df928f000ea68e967b,
title = "Human topoisomerase IIIalpha is a single-stranded DNA decatenase that is stimulated by BLM and RMI1",
abstract = "Human topoisomerase IIIalpha is a type IA DNA topoisomerase that functions with BLM and RMI1 to resolve DNA replication and recombination intermediates. BLM, human topoisomerase IIIalpha, and RMI1 catalyze the dissolution of double Holliday junctions into noncrossover products via a strand-passage mechanism. We generated single-stranded catenanes that resemble the proposed dissolution intermediate recognized by human topoisomerase IIIalpha. We demonstrate that human topoisomerase IIIalpha is a single-stranded DNA decatenase that is specifically stimulated by the BLM-RMI1 pair. In addition, RMI1 interacts with human topoisomerase IIIalpha, and the interaction is required for the stimulatory effect of RMI1 on decatenase activity. Our data provide direct evidence that human topoisomerase IIIalpha functions as a decatenase with the assistance of BLM and RMI1 to facilitate the processing of homologous recombination intermediates without crossing over as a mechanism to preserve genome integrity.",
author = "Jay Yang and Bachrati, {Csanad Z} and Jiongwen Ou and Hickson, {Ian David} and Brown, {Grant W}",
year = "2010",
doi = "10.1074/jbc.M110.123216",
language = "English",
volume = "285",
pages = "21426--36",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology, Inc.",
number = "28",

}

RIS

TY - JOUR

T1 - Human topoisomerase IIIalpha is a single-stranded DNA decatenase that is stimulated by BLM and RMI1

AU - Yang, Jay

AU - Bachrati, Csanad Z

AU - Ou, Jiongwen

AU - Hickson, Ian David

AU - Brown, Grant W

PY - 2010

Y1 - 2010

N2 - Human topoisomerase IIIalpha is a type IA DNA topoisomerase that functions with BLM and RMI1 to resolve DNA replication and recombination intermediates. BLM, human topoisomerase IIIalpha, and RMI1 catalyze the dissolution of double Holliday junctions into noncrossover products via a strand-passage mechanism. We generated single-stranded catenanes that resemble the proposed dissolution intermediate recognized by human topoisomerase IIIalpha. We demonstrate that human topoisomerase IIIalpha is a single-stranded DNA decatenase that is specifically stimulated by the BLM-RMI1 pair. In addition, RMI1 interacts with human topoisomerase IIIalpha, and the interaction is required for the stimulatory effect of RMI1 on decatenase activity. Our data provide direct evidence that human topoisomerase IIIalpha functions as a decatenase with the assistance of BLM and RMI1 to facilitate the processing of homologous recombination intermediates without crossing over as a mechanism to preserve genome integrity.

AB - Human topoisomerase IIIalpha is a type IA DNA topoisomerase that functions with BLM and RMI1 to resolve DNA replication and recombination intermediates. BLM, human topoisomerase IIIalpha, and RMI1 catalyze the dissolution of double Holliday junctions into noncrossover products via a strand-passage mechanism. We generated single-stranded catenanes that resemble the proposed dissolution intermediate recognized by human topoisomerase IIIalpha. We demonstrate that human topoisomerase IIIalpha is a single-stranded DNA decatenase that is specifically stimulated by the BLM-RMI1 pair. In addition, RMI1 interacts with human topoisomerase IIIalpha, and the interaction is required for the stimulatory effect of RMI1 on decatenase activity. Our data provide direct evidence that human topoisomerase IIIalpha functions as a decatenase with the assistance of BLM and RMI1 to facilitate the processing of homologous recombination intermediates without crossing over as a mechanism to preserve genome integrity.

U2 - 10.1074/jbc.M110.123216

DO - 10.1074/jbc.M110.123216

M3 - Journal article

C2 - 20445207

VL - 285

SP - 21426

EP - 21436

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 28

ER -

ID: 20971267