Detection of Ultrafine Anaphase Bridges

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Detection of Ultrafine Anaphase Bridges. / Bizard, Anna H; Nielsen, Christian F; Hickson, Ian D.

Genome Instability: Methods and Protocols. Vol. 1672 Springer, 2018. p. 495-508 (Methods in molecular biology (Clifton, N.J.)).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Bizard, AH, Nielsen, CF & Hickson, ID 2018, Detection of Ultrafine Anaphase Bridges. in Genome Instability: Methods and Protocols. vol. 1672, Springer, Methods in molecular biology (Clifton, N.J.), pp. 495-508. https://doi.org/10.1007/978-1-4939-7306-4_33

APA

Bizard, A. H., Nielsen, C. F., & Hickson, I. D. (2018). Detection of Ultrafine Anaphase Bridges. In Genome Instability: Methods and Protocols (Vol. 1672, pp. 495-508). Springer. Methods in molecular biology (Clifton, N.J.) https://doi.org/10.1007/978-1-4939-7306-4_33

Vancouver

Bizard AH, Nielsen CF, Hickson ID. Detection of Ultrafine Anaphase Bridges. In Genome Instability: Methods and Protocols. Vol. 1672. Springer. 2018. p. 495-508. (Methods in molecular biology (Clifton, N.J.)). https://doi.org/10.1007/978-1-4939-7306-4_33

Author

Bizard, Anna H ; Nielsen, Christian F ; Hickson, Ian D. / Detection of Ultrafine Anaphase Bridges. Genome Instability: Methods and Protocols. Vol. 1672 Springer, 2018. pp. 495-508 (Methods in molecular biology (Clifton, N.J.)).

Bibtex

@inbook{d58b8132b2c5412a8dc1b8e2c7a315e7,
title = "Detection of Ultrafine Anaphase Bridges",
abstract = "Ultrafine anaphase bridges (UFBs) are thin DNA threads linking the separating sister chromatids in the anaphase of mitosis. UFBs are thought to form when topological DNA entanglements between two chromatids are not resolved prior to anaphase onset. In contrast to other markers of defective chromosome segregation, UFBs cannot be detected by direct staining of the DNA, but instead can be detected using immunofluorescence-based approaches. Due to the fact that they are short-lived and fragile in nature, UFBs can be challenging to detect. In this chapter, we describe methods that have been optimized for successful detection of UFBs. We also provide guidelines for the optimization of UFBs detection depending on the antibody and the cell line to be used.",
author = "Bizard, {Anna H} and Nielsen, {Christian F} and Hickson, {Ian D}",
year = "2018",
doi = "10.1007/978-1-4939-7306-4_33",
language = "English",
isbn = "978-1-4939-7305-7",
volume = "1672",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Springer",
pages = "495--508",
booktitle = "Genome Instability",
address = "Switzerland",

}

RIS

TY - CHAP

T1 - Detection of Ultrafine Anaphase Bridges

AU - Bizard, Anna H

AU - Nielsen, Christian F

AU - Hickson, Ian D

PY - 2018

Y1 - 2018

N2 - Ultrafine anaphase bridges (UFBs) are thin DNA threads linking the separating sister chromatids in the anaphase of mitosis. UFBs are thought to form when topological DNA entanglements between two chromatids are not resolved prior to anaphase onset. In contrast to other markers of defective chromosome segregation, UFBs cannot be detected by direct staining of the DNA, but instead can be detected using immunofluorescence-based approaches. Due to the fact that they are short-lived and fragile in nature, UFBs can be challenging to detect. In this chapter, we describe methods that have been optimized for successful detection of UFBs. We also provide guidelines for the optimization of UFBs detection depending on the antibody and the cell line to be used.

AB - Ultrafine anaphase bridges (UFBs) are thin DNA threads linking the separating sister chromatids in the anaphase of mitosis. UFBs are thought to form when topological DNA entanglements between two chromatids are not resolved prior to anaphase onset. In contrast to other markers of defective chromosome segregation, UFBs cannot be detected by direct staining of the DNA, but instead can be detected using immunofluorescence-based approaches. Due to the fact that they are short-lived and fragile in nature, UFBs can be challenging to detect. In this chapter, we describe methods that have been optimized for successful detection of UFBs. We also provide guidelines for the optimization of UFBs detection depending on the antibody and the cell line to be used.

UR - https://link.springer.com/protocol/10.1007%2F978-1-4939-7306-4_43

U2 - 10.1007/978-1-4939-7306-4_33

DO - 10.1007/978-1-4939-7306-4_33

M3 - Book chapter

C2 - 29043644

SN - 978-1-4939-7305-7

VL - 1672

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 495

EP - 508

BT - Genome Instability

PB - Springer

ER -

ID: 190437306