Early Growth Response Genes Increases Rapidly After Mechanical Overloading and Unloading in Tendon Constructs
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Early Growth Response Genes Increases Rapidly After Mechanical Overloading and Unloading in Tendon Constructs. / Herchenhan, Andreas; Dietrich-Zagonel, Franciele; Schjerling, Peter; Kjaer, Michael; Eliasson, Pernilla.
In: Journal of Orthopaedic Research, Vol. 38, No. 1, 2020, p. 173-181.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Early Growth Response Genes Increases Rapidly After Mechanical Overloading and Unloading in Tendon Constructs
AU - Herchenhan, Andreas
AU - Dietrich-Zagonel, Franciele
AU - Schjerling, Peter
AU - Kjaer, Michael
AU - Eliasson, Pernilla
PY - 2020
Y1 - 2020
N2 - Tendon cells exist in a dense extracellular matrix and mechanical loading is important for the strength development of this matrix. We therefore use a three‐dimensional (3D) culture system for tendon formation in vitro. The objectives of this study were to elucidate the temporal expression of tendon‐related genes during the formation of artificial tendons in vitro and to investigate if early growth response‐1 (EGR1), EGR2, FOS, and cyclooxygenase‐1 and ‐2 (PTGS1 and PTGS2) are sensitive to mechanical loading. First, we studied messenger RNA (mRNA) levels of several tendon‐related genes during formation of tendon constructs. Second, we studied the mRNA levels of, for example, EGR1 and EGR2 after different degrees of loading; dynamic physiologic‐range loading (2.5% strain), dynamic overloading (approximately 10% strain), or tension release. The gene expression for tendon‐related genes (i.e., EGR2, MKX, TNMD, COL3A1) increased with time after seeding into this 3D model. EGR1, EGR2, FOS, PTGS1, and PTGS2 did not respond to physiologic‐range loading. But overloading (and tension release) lead to elevated levels of EGR1 and EGR2 (p ≤ 0.006). FOS and PTGS2 were increased after overloading (both p < 0.007) but not after tension release (p = 0.06 and 0.08). In conclusion, the expression of tendon‐related genes increases during the formation of artificial tendons in vitro, including EGR2. Furthermore, the gene expression of EGR1 and EGR2 in human tendon cells appear to be sensitive to overloading and unloading but did not respond to the single episode of physiologic‐range loading. These findings could be helpful for the understanding of tendon tensional homeostasis
AB - Tendon cells exist in a dense extracellular matrix and mechanical loading is important for the strength development of this matrix. We therefore use a three‐dimensional (3D) culture system for tendon formation in vitro. The objectives of this study were to elucidate the temporal expression of tendon‐related genes during the formation of artificial tendons in vitro and to investigate if early growth response‐1 (EGR1), EGR2, FOS, and cyclooxygenase‐1 and ‐2 (PTGS1 and PTGS2) are sensitive to mechanical loading. First, we studied messenger RNA (mRNA) levels of several tendon‐related genes during formation of tendon constructs. Second, we studied the mRNA levels of, for example, EGR1 and EGR2 after different degrees of loading; dynamic physiologic‐range loading (2.5% strain), dynamic overloading (approximately 10% strain), or tension release. The gene expression for tendon‐related genes (i.e., EGR2, MKX, TNMD, COL3A1) increased with time after seeding into this 3D model. EGR1, EGR2, FOS, PTGS1, and PTGS2 did not respond to physiologic‐range loading. But overloading (and tension release) lead to elevated levels of EGR1 and EGR2 (p ≤ 0.006). FOS and PTGS2 were increased after overloading (both p < 0.007) but not after tension release (p = 0.06 and 0.08). In conclusion, the expression of tendon‐related genes increases during the formation of artificial tendons in vitro, including EGR2. Furthermore, the gene expression of EGR1 and EGR2 in human tendon cells appear to be sensitive to overloading and unloading but did not respond to the single episode of physiologic‐range loading. These findings could be helpful for the understanding of tendon tensional homeostasis
KW - tenocytes
KW - mechanical loading
KW - tension release
KW - EGR1
KW - EGR2
U2 - 10.1002/jor.24513
DO - 10.1002/jor.24513
M3 - Journal article
C2 - 31692087
VL - 38
SP - 173
EP - 181
JO - Journal of Orthopaedic Research
JF - Journal of Orthopaedic Research
SN - 0736-0266
IS - 1
ER -
ID: 257969397