A Molecular Toolbox to Engineer Site-Specific DNA Replication Perturbation

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

A Molecular Toolbox to Engineer Site-Specific DNA Replication Perturbation. / Larsen, Nicolai B; Hickson, Ian D; Mankouri, Hocine W.

Genome Instability: Methods and Protocols. Vol. 1672 Springer, 2018. p. 295-309 (Methods in molecular biology (Clifton, N.J.)).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Larsen, NB, Hickson, ID & Mankouri, HW 2018, A Molecular Toolbox to Engineer Site-Specific DNA Replication Perturbation. in Genome Instability: Methods and Protocols. vol. 1672, Springer, Methods in molecular biology (Clifton, N.J.), pp. 295-309. https://doi.org/10.1007/978-1-4939-7306-4_20

APA

Larsen, N. B., Hickson, I. D., & Mankouri, H. W. (2018). A Molecular Toolbox to Engineer Site-Specific DNA Replication Perturbation. In Genome Instability: Methods and Protocols (Vol. 1672, pp. 295-309). Springer. Methods in molecular biology (Clifton, N.J.) https://doi.org/10.1007/978-1-4939-7306-4_20

Vancouver

Larsen NB, Hickson ID, Mankouri HW. A Molecular Toolbox to Engineer Site-Specific DNA Replication Perturbation. In Genome Instability: Methods and Protocols. Vol. 1672. Springer. 2018. p. 295-309. (Methods in molecular biology (Clifton, N.J.)). https://doi.org/10.1007/978-1-4939-7306-4_20

Author

Larsen, Nicolai B ; Hickson, Ian D ; Mankouri, Hocine W. / A Molecular Toolbox to Engineer Site-Specific DNA Replication Perturbation. Genome Instability: Methods and Protocols. Vol. 1672 Springer, 2018. pp. 295-309 (Methods in molecular biology (Clifton, N.J.)).

Bibtex

@inbook{f291726adb39455b92f282bfb6bcc8bc,
title = "A Molecular Toolbox to Engineer Site-Specific DNA Replication Perturbation",
abstract = "Site-specific arrest of DNA replication is a useful tool for analyzing cellular responses to DNA replication perturbation. The E. coli Tus-Ter replication barrier can be reconstituted in eukaryotic cells as a system to engineer an unscheduled collision between a replication fork and an {"}alien{"} impediment to DNA replication. To further develop this system as a versatile tool, we describe a set of reagents and a detailed protocol that can be used to engineer Tus-Ter barriers into any locus in the budding yeast genome. Because the Tus-Ter complex is a bipartite system with intrinsic DNA replication-blocking activity, the reagents and protocols developed and validated in yeast could also be optimized to engineer site-specific replication fork barriers into other eukaryotic cell types.",
author = "Larsen, {Nicolai B} and Hickson, {Ian D} and Mankouri, {Hocine W}",
year = "2018",
doi = "10.1007/978-1-4939-7306-4_20",
language = "English",
isbn = "978-1-4939-7305-7",
volume = "1672",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Springer",
pages = "295--309",
booktitle = "Genome Instability",
address = "Switzerland",

}

RIS

TY - CHAP

T1 - A Molecular Toolbox to Engineer Site-Specific DNA Replication Perturbation

AU - Larsen, Nicolai B

AU - Hickson, Ian D

AU - Mankouri, Hocine W

PY - 2018

Y1 - 2018

N2 - Site-specific arrest of DNA replication is a useful tool for analyzing cellular responses to DNA replication perturbation. The E. coli Tus-Ter replication barrier can be reconstituted in eukaryotic cells as a system to engineer an unscheduled collision between a replication fork and an "alien" impediment to DNA replication. To further develop this system as a versatile tool, we describe a set of reagents and a detailed protocol that can be used to engineer Tus-Ter barriers into any locus in the budding yeast genome. Because the Tus-Ter complex is a bipartite system with intrinsic DNA replication-blocking activity, the reagents and protocols developed and validated in yeast could also be optimized to engineer site-specific replication fork barriers into other eukaryotic cell types.

AB - Site-specific arrest of DNA replication is a useful tool for analyzing cellular responses to DNA replication perturbation. The E. coli Tus-Ter replication barrier can be reconstituted in eukaryotic cells as a system to engineer an unscheduled collision between a replication fork and an "alien" impediment to DNA replication. To further develop this system as a versatile tool, we describe a set of reagents and a detailed protocol that can be used to engineer Tus-Ter barriers into any locus in the budding yeast genome. Because the Tus-Ter complex is a bipartite system with intrinsic DNA replication-blocking activity, the reagents and protocols developed and validated in yeast could also be optimized to engineer site-specific replication fork barriers into other eukaryotic cell types.

U2 - 10.1007/978-1-4939-7306-4_20

DO - 10.1007/978-1-4939-7306-4_20

M3 - Book chapter

C2 - 29043631

SN - 978-1-4939-7305-7

VL - 1672

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 295

EP - 309

BT - Genome Instability

PB - Springer

ER -

ID: 190852365