Differentially activated macrophages orchestrate myogenic precursor cell fate during human skeletal muscle regeneration
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Differentially activated macrophages orchestrate myogenic precursor cell fate during human skeletal muscle regeneration. / Saclier, Marielle; Yacoub-Youssef, Houda; Mackey, Abigail; Arnold, Ludovic; Ardjoune, Hamida; Magnan, Mélanie; Sailhan, Frédéric; Chelly, Jamel; Pavlath, Grace K; Mounier, Rémi; Kjaer, Michael; Chazaud, Bénédicte.
In: Stem Cells, Vol. 31, No. 2, 02.2013, p. 384-96.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Differentially activated macrophages orchestrate myogenic precursor cell fate during human skeletal muscle regeneration
AU - Saclier, Marielle
AU - Yacoub-Youssef, Houda
AU - Mackey, Abigail
AU - Arnold, Ludovic
AU - Ardjoune, Hamida
AU - Magnan, Mélanie
AU - Sailhan, Frédéric
AU - Chelly, Jamel
AU - Pavlath, Grace K
AU - Mounier, Rémi
AU - Kjaer, Michael
AU - Chazaud, Bénédicte
N1 - Copyright © 2012 AlphaMed Press.
PY - 2013/2
Y1 - 2013/2
N2 - Macrophages (MPs) exert either beneficial or deleterious effects on tissue repair, depending on their activation/polarization state. They are crucial for adult skeletal muscle repair, notably by acting on myogenic precursor cells. However, these interactions have not been fully characterized. Here, we explored both in vitro and in vivo, in human, the interactions of differentially activated MPs with myogenic precursor cells (MPCs) during adult myogenesis and skeletal muscle regeneration. We showed in vitro that through the differential secretion of cytokines and growth factors, proinflammatory MPs inhibited MPC fusion while anti-inflammatory MPs strongly promoted MPC differentiation by increasing their commitment into differentiated myocytes and the formation of mature myotubes. Furthermore, the in vivo time course of expression of myogenic and MP markers was studied in regenerating human healthy muscle after damage. We observed that regenerating areas containing proliferating MPCs were preferentially associated with MPs expressing proinflammatory markers. In the same muscle, regenerating areas containing differentiating myogenin-positive MPCs were preferentially coupled to MPs harboring anti-inflammatory markers. These data demonstrate for the first time in human that MPs sequentially orchestrate adult myogenesis during regeneration of damaged skeletal muscle. These results support the emerging concept that inflammation, through MP activation, controls stem cell fate and coordinates tissue repair.
AB - Macrophages (MPs) exert either beneficial or deleterious effects on tissue repair, depending on their activation/polarization state. They are crucial for adult skeletal muscle repair, notably by acting on myogenic precursor cells. However, these interactions have not been fully characterized. Here, we explored both in vitro and in vivo, in human, the interactions of differentially activated MPs with myogenic precursor cells (MPCs) during adult myogenesis and skeletal muscle regeneration. We showed in vitro that through the differential secretion of cytokines and growth factors, proinflammatory MPs inhibited MPC fusion while anti-inflammatory MPs strongly promoted MPC differentiation by increasing their commitment into differentiated myocytes and the formation of mature myotubes. Furthermore, the in vivo time course of expression of myogenic and MP markers was studied in regenerating human healthy muscle after damage. We observed that regenerating areas containing proliferating MPCs were preferentially associated with MPs expressing proinflammatory markers. In the same muscle, regenerating areas containing differentiating myogenin-positive MPCs were preferentially coupled to MPs harboring anti-inflammatory markers. These data demonstrate for the first time in human that MPs sequentially orchestrate adult myogenesis during regeneration of damaged skeletal muscle. These results support the emerging concept that inflammation, through MP activation, controls stem cell fate and coordinates tissue repair.
U2 - 10.1002/stem.1288
DO - 10.1002/stem.1288
M3 - Journal article
C2 - 23169615
VL - 31
SP - 384
EP - 396
JO - Stem Cells
JF - Stem Cells
SN - 1066-5099
IS - 2
ER -
ID: 47292387